TABLE 9.
sgRNA in silico and in vivo efficiency, compared to the final efficiency observed in the number of generated mutants.
| In silico efficiency | No of heterozygotes | ||
| CRIPOR prediction | In vivo efficiency | in F1 generation/ | |
| Guide | score | % of mosaic eggs | No of G0 parents |
| N-cry2 2 | 44 | †nd | 0 |
| N-cry2 3 | 32 | †nd | 0 |
| N-cry2 8 | 57 | †nd | 7/6 |
| C-cry2 188 | 38 | 4.0 | †nd |
| C-cry2 194 | 25 | 38.0 | 11/5 |
| C-cry2 196 | 30 | 28.0 | †nd |
| tim 1448 | 61 | 61.3 | †nd |
| tim 1481 | 54 | 23.1 | †nd |
| tim 1517 | 47 | 4.5 | †nd |
| tim 1587 | 50 | 36.0 | 64/16 |
| tim 2114 | 72 | 25.0 | 14/6 |
| tim 2193 | 46 | 0 | †nd |
| perS 1 | 32 | †nd | 2/2 |
| perS 4 | 52 | †nd | 1/1 |
| perSLIH 1 | 24 | 22.0 | 0/0 |
| perSLIH 2 | 40 | 9.5 | †nd |
| perSLIH 3 | 50 | 10.0 | 0/0 |
| perSLIH 4 | 67 | 23.5 | 19/2 |
| perSLIH 5 | 54 | 5.2 | †nd |
| perL 1 | 49 | 0 | †nd |
| perL 2 | 33 | 65.0 | 30/3 |
| perl 3 | 33 | 47.0 | 56/7 |
| PDF 1 crRNA | 23 | 63.6 | 21/4 |
| PDF 1 | 23 | 5.2 | 1/1 |
| PDF 2 | 38 | 78.2 | 80/5 |
| PDF 3 | 32 | 19.2 | †nd |
| TEFL 1 | 42 | 0 | †nd |
| TEFL 2 | 34 | 3.7 | 2/2 |
| TEFL 3 | 61 | 34 | 22/7 |
| TEFL 4 | 31 | 0 | †nd |
| TEFL 5 | 45 | 19 | 1/1 |
| TEFL 7 | 32 | 6.2 | †nd |
| TEFL 8 | 33 | 16 | †nd |
| TEFL 9 | 50 | 0 | †nd |
In vivo efficiency column shows % of eggs displaying mosaicism when injected with particular sgRNA. The last column shows the % of heterozygotes of the total number of F1 individuals screened. •nd, not determined.