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ThT fluorescence plots showing the fibril formation process with time for HIF at pH 1.6, 25 °C, 0 M urea, 0.02 M NaCl (−■−); pH 1.6, 25 °C, 1 M urea, 1 M NaCl (−●−); pH 1.6, 60 °C, 0 M urea, 1 M NaCl (−▲−); pH 1.6, 60 °C, 1 M urea, 0.02 M NaCl (−▼−); pH 5, 25°C, 0 M urea, 1 M NaCl (−⧫−); pH 5, 25 °C, 1 M urea, 0.02 M NaCl (−◀−); pH 5, 60 °C, 0 M urea, 0.02 M NaCl (−▶−); pH 5, 60 °C 1 M urea, 1 M NaCl (−⬢−).
