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. 2019 Jul 10;15(7):e1008253. doi: 10.1371/journal.pgen.1008253

Fig 1. Knockdown of Cyclin A induces endoreplication cycles.

Fig 1

(A) Flow cytometry of DNA content in propidium iodide labeled S2 cells 96 hours after treatment with either GFP dsRNA (control) or CycA dsRNA. (B) Quantification of the ploidy classes after GFP or CycA dsRNA treatment. Mean and S.E.M. for N = 3, *—p <0.05 comparing each CycA dsRNA ploidy class with the corresponding ploidy class in control GFP dsRNA treated cells. (C) qRT-PCR analysis of select Myb and E2F1 target gene expression in CycA dsRNA iECs. Normalized mRNA is the ratio of mRNA levels in CycA dsRNA divided by control GFP dsRNA cells (N = 3, mean and S.E.M., *—p < 0.05, **—p < 0.01, ns—not significant).