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. 2019 Jul 10;15(7):e1008253. doi: 10.1371/journal.pgen.1008253

Fig 6. Knockdown of a CycA—Myb—AurB network induces endoreplication in ovarian follicle cells.

Fig 6

(A-L) Clonal knockdown in follicle cells. FLP-On of GAL4 expression was induced by heat treatment of adult females of genotype hsp70-FLP, Act>cd2>Gal4, UAS-mRFP and the indicated UAS-dsRNA. mRFP positive clones were analyzed in stage 6 egg chambers three days later by labeling with DAPI and measuring nuclear size and total fluorescence intensity (DNA content). Red outlines in A-L indicate clone boundaries. (M) Quantification of number of cells per clone. RFP expressing cells were counted from at least four independent clones. More clones were counted in samples with fewer cells / clone (mean and S.D. for N ≥ 4 clones, **—p<0.01 relative to wild type control clones). (N) Quantification of nuclear area and DAPI intensity. The nuclear area (blue dots) and DAPI intensity (orange dots) of single cells in a clone (RFP+) were measured and divided by the mean nuclear area and DAPI intensity of wild type cells (RFP-) in the same egg chamber (mean and S.D. for N ≥ 3 clones, *—p<0.05, **—p<0.01, ns—not significant relative to wild type, control cells).