A, Sample western blot images showing changes in protein expression of supraoptic nucleus (SON) punches. mCherry protein expression images shows successful injections at SON B, Quantification of phosphorylated tyrosine receptor kinase B (TrkB) (normalised to total TrkB). Data are the mean ± SEM. *P < 0.05 vs all other groups.+P < 0.05 vs salt scrambled (SCR). C, Phosphorylated KCC2 normalised to GAPDH. *P < 0.05 vs all other groups. Groups: euhydrated rats injected with SCR virus (Eu SCR, n = 6); euhydrated rats injected with shBDNF virus (Eu shBDNF, n = 6); salt loaded rats injected with SCR virus (Salt SCR, n = 8) and salt loaded rats injected with shBDNF virus (Salt shBDNF, n = 8)