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. 2019 Jul 22;10(8):558. doi: 10.1038/s41419-019-1797-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Immunofluorescence analysis of TGFB1, TGFB2, TGFB3, TGFBR1, and TGFBR2 in the ovaries isolated from eCG-primed mice. The images are representative of three images captured. The small white boxes indicate the enlarged areas as shown in the following images. Scale bars: 200 μm. b Comparison of steady-state levels of Tgfb1, Tgfb2, Tgfb3, Tgfbr1, and Tgfbr2 mRNA in MGCs, cumulus cells (CCs) and oocytes isolated from eCG-primed mice. c Representative western blotting of TGFB1, TGFB2, TGFB3, TGFBR1, and TGFBR2 in MGCs, CCs and, oocytes isolated from eCG-primed mice. GAPDH was used as a loading control. Bars indicate the mean ± SEM of three independent replicates. Values not indicated by the same letter are significantly different (P < 0.05)