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. 2019 Jul 22;10(8):558. doi: 10.1038/s41419-019-1797-5

Fig. 2. Gonadotropins regulate the expression of TGF-β signaling molecules in MGCs.

Fig. 2

MGCs were isolated from prepubertal mice, eCG-primed mice and eCG-primed mice followed by hCG as indicated in the figures. a Quantitative RT-PCR analysis of the gene expression of Tgfb1, Tgfb3, Tgfbr1, and Tgfbr2 in MGCs. b Western blotting analysis of the protein levels of TGFB1, TGFB3, TGFBR1, and TGFBR2 in MGCs. c Quantitative RT-PCR analysis of the gene expression of Smad2, Smad3, and Smad4 in MGCs. d Western blotting analysis of the protein levels of SMAD2/3, SMAD4, and phosphorylated SMAD2/3 (p-SMAD2/3) in MGCs. The blots shown are representative of three images captured in b, d. GAPDH was used as a loading control in b, d. Bars indicate the mean ± SEM of three independent replicates. Values not indicated by the same letter are significantly different (P < 0.05)