Skip to main content
. 2019 Jul 22;10(8):558. doi: 10.1038/s41419-019-1797-5

Fig. 4. TGF-β promotes NPPC expression and maintains oocyte meiotic arrest.

Fig. 4

a–c MGCs were collected from prepubertal mice and cultured for 24 h in cell culture medium supplemented with 10 ng/ml TGFB1, 50 ng/ml TGFB2, 10 ng/ml TGFB3, TGF-β (including 10 ng/ml TGFB1, 50 ng/ml TGFB2, 10 ng/ml TGFB3), 1 μmol/l SD208 (SD), and/or 10 ng/ml FSH. NPPC and/or NPR2 mRNA and protein levels were detected by qRT-PCR (a), western blotting (b) and a luciferase immunoassay (c), respectively. The blots shown are representative of three images captured. GAPDH was used as a loading control. d Effect of TGF-β on oocyte meiotic resumption of COCs. MGCs were cultured in the medium supplemented with TGF-β, SD208, and/or FSH for 24 h, and then COCs isolated from eCG-primed mice were cocultured with MGCs for 1, 2, 3, and 4 h. n = 30 for each treatment across all three replicates. **P ˂ 0.01 and ***P ˂ 0.001 compared with corresponding control. e Effect of TGF-β on the meiotic resumption of oocytes within follicles. Large antral follicles isolated from eCG-primed mice were cultured in the medium supplemented with TGF-β, and/or SD208 for 4 h. n = 30 for each treatment across all three replicates. Bars indicate the mean ± SEM of three independent replicates. Values not indicated by the same letter are significantly different (P < 0.05)