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. 2019 Jul 22;9:10591. doi: 10.1038/s41598-019-47033-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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USP10 does not interact with Tau. (a) HT22 cells were transfected with Tau (Tau4R1N) plasmid along with HA-USP10 and/or FLAG-TIA1 plasmid using Lipofectamine 2000. Prepared cell lysates were immunoprecipitated with an anti-HA antibody, and the amounts of Tau, FLAG-TIA1 and HA-USP10 were analyzed by Western blotting using respective antibodies. (b–e) HeLa cells were transfected with HA-USP10 plasmid along either with Tau3R1N, Tau4R1N or Tau3R1N/K317M plasmid by lipofection using Lipofectamine 2000. At 48 h after transfection, whole cell lysates (b,c), NP-40-soluble fractions and NP-40-insoluble fractions (d,e) were prepared and characterized by Western blotting using anti-Tau (TAU-5), anti-phosphorylated Tau (pTau) (AT8), anti-HA, anti-TIA1, anti-α-tubulin and anti-β-actin antibodies.