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. 2019 Jul 22;9:10552. doi: 10.1038/s41598-019-47156-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) The USF signals acquired at x = 0.00 mm (i.e., FU was focused on center of the tube) with different Vpp (i.e., Vpp = 70 mV, 100 mV, 130 mV and 160 mV). In the left plot, it shows the USF signal strength increased with Vpp. In the right plot, it shows the normalized signal strengths over time: USF signal duration also increased with the increase of Vpp. (b) 2D USF image representing the relationship between image profile and FU power. (c) Relationship between SNR of imaged tube and FU power. (d) Relationship between FWHM of imaged tube and FU power.