Fig. 6.

Time of bud initiation changes developmental time. Changing developmental time by application of TGF-β receptor inhibitors. a Schematic representation correlating maximum size and head inhibition gradient. Larger Hydra lines have a higher head inhibition potential, thereby respond less to ALP, and extend in developmental time (ΔT₁). b K02288 antedated the time point of bud initiation in developmental time, eventually resulting in smaller maximum size. Points show single samples, line represents the mean. n = 29 polyps (DMSO), 27 polyps (K02288). c LDN-193189 suppressed bud initiation, leading to longer ΔT₁ and larger maximum size. n = 29, ***p ≤ 0.001, U-test