. 2019 Jul 22;9:10548. doi: 10.1038/s41598-019-47114-7

Table 1.

CYP450 activities in 3D HepaRG spheroids.

CYP	Substrate	Metabolite	CYP activity (pmol/min-million cells)
CYP	Substrate	Metabolite	Control	OME	PB	RIF
CYP1A2	Phenacetin	Acetaminophen	ND	ND	ND	ND
CYP2B6	Bupropion	OH-bupropion	<LOQ^a	<LOQ^b	<LOQ^b	<LOQ^b
CYP2C9	Diclofenac	OH-diclofenac	1.39 ± 0.54	1.27 ± 0.46	2.66 ± 2.03	2.38 ± 1.79*
CYP3A4	Midazolam	1′OH-midazolam	0.41 ± 0.19	0.08 ± 0.06	1.35 ± 0.96	1.93 ± 1.54

ND: not detected, <LOD corresponding to 1/3 of LOQ (Limit of Quantification (µM)), ^a0.5 µM corresponding to 17.36 pmol/min-million cells; ^b0.01 µM corresponding to 0.35 pmol/min-million cells. OME = omeprazole, PB = phenobarbital, RIF = rifampicin, *p < 0.05.