Fig. 10.

The adult heart does not activate developmental vascular regulatory pathways after MI. a–c Permanent ligation of the left anterior descending coronary artery was performed on transgenic adult male mice to induce MI. Representative sections through HLX-3:lacZ (a, n = 4 biologically independent animals), Dll4-12:lacZ (b, n = 5 biologically independent animals), and Ephb4-2:lacZ (c, n = 4 biologically independent animals) hearts collected 7 days after MI surgery compared to sham-operated controls. Additional time points after MI showed similar responses and can be seen in Supplementary Fig. 19, cumulative n values are n = 12 biologically independent animals for HLX-3:lacZ, n = 13 biologically independent animals for Dll4-12:lacZ and n = 12 biologically independent animals for Ephb4-2:lacZ. Infarct regions are indicated by grey dashed line, transgene expression is detected by X-gal staining (blue). Magnified views are shown of regions within the injury (box 1), at the injury border zone (box 2) and remote from the injury (box 3), and can be compared to sham-operated day seven controls. lv, left ventricle; rv, right ventricle. Black scale bars represent 500 µm. d Schematic summarising activity patterns of different vascular regulatory pathways in the coronary vasculature during development and after ischaemic injury