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. 2019 Jul 22;9:10602. doi: 10.1038/s41598-019-46968-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inflammatory gene expression during POI is reduced in IL-1α and IL-1β deficient mice. Wt, IL-1α^−/− and IL-1β^−/− mice underwent IM. Subsequently, (A–E) mRNA expression of indicated genes was quantified in small bowel ME. Statistical analysis: 1-way ANOVA and Bonferroni test. (n = 3 for CTLs and n = 5 for IM per group). (F,G) Enteric glia cell cultures from wt and IL-1R1^−/− mice were stimulated with IL-1α or IL-1β for 24 h and MCP-1 or IL-6 mRNA levels were analysed. Statistical analyses: two-way ANOVA followed by Bonferroni posthoc test. (n = 5 per group). CTL = (A–E) untreated animals or (F,G) vehicle-treated cells.