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. 2019 Jul 22;9:10580. doi: 10.1038/s41598-019-47048-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a–d) Representative 2D slices of healthy myometrium (Ctr) and pathologic (L) tissues. (a,b) Patient 1 (P1): healthy myometrium (a: P1-Ctr) and leiomyoma (b: P1-L) tissues; (c,d) Patient 2 (P2): healthy myometrium (c: P2-Ctr) and leiomyoma (d: P2-L) tissues. Morphological dissimilarities were found not only between Ctr and L samples of the same patient and between pathologic tissues of the two patients (P1-L vs. P2-L) but also between the healthy tissues of the two patients (P1-Ctr vs. P2-Ctr). (e,f) Representative 3D volumes of healthy myometrium (e: P1-Ctr) and pathologic (f: P1-L) tissues: all the tissues but the collagen phase have been made virtually transparent. The bright spots that we found in all the samples were due, most likely, to the presence of blood clots residues, not removed by washing because of the structure tortuosity.