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. 2019 Jul 22;9:10603. doi: 10.1038/s41598-019-47112-9

Figure 1.

Figure 1

CG4 cells display an OPC-like morphology in the undifferentiated state (A) and oligodendrocyte-like phenotype in the differentiated state (B). PV (Pvalb) transcript levels were determined by semi-quantitative PCR after 30 cycles (upper) and by RT-qPCR (lower), where Cypa was used as reference gene (C,D). PV expression levels were determined by Western blot analyses, where GAPDH signals were used as loading control (E,F). The morphological features of LV-infected CG4 cells (GFP, PV, shPV) appeared similar to the control (C) in both undifferentiated OPC-like and differentiated OLG-like states (G,H).