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. 2019 Jul 22;9:10603. doi: 10.1038/s41598-019-47112-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth properties are unaffected after PV upregulation. Confluence analysis of undifferentiated CG4 cells (A) or differentiated CG4 cells (B) with representative images (C-CG4 cells) taken by the IncuCyte system 1 h, 30 h and 50 h after plating showed no significant alterations in the growth curve slopes of C-, GFP- and PV-CG4 cells in the undifferentiated state (C,E) or in the differentiated state (D,F).