Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 22;9:10603. doi: 10.1038/s41598-019-47112-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PV expression affected “wound closure” capacity as assessed by a scratch wound assay using undifferentiated C-, GFP- and PV-CG4 cells (A) and the same differentiated CG4 cell lines (B). The wound closure capacity was evaluated by calculating the AUC of the relative wound density plotted over time, which was normalized to the control (C-CG4 cells) and averaged per condition. In the undifferentiated OPC state PV-CG4 cells displayed slightly decreased AUC values (relative wound density over time) when compared to C-CG4 cells (C), suggesting a reduced wound closure capacity in PV-overexpressing CG4 cells in the undifferentiated state. Similar differences were also present in CG4 cells in the differentiated state (E). Single-cell tracking from video sequences revealed no significant differences in the cell velocity (µm/min) of CG4 cells, in both the undifferentiated OPC-like (D) and differentiated OLG-like states (F). Error bars represent SEM (ns: not significant; p value > 0.05, *p < 0.05, **p < 0.001).