Table 1.
Depth of z-stacks, indicative of imaging depth, achieved in confocal microscopy with different immersion liquids, PBS, glycerol, and ScaleView-A2.
| Depth achieved (μm) | ||||
|---|---|---|---|---|
| Propidium Iodide | Hoechst 33342 | |||
| Mean | SD (N) | Mean | SD (N) | |
| PBS (n ≈1.33) | 220 | 25 (8) | 188 | 20 (6) |
| Glycerol (n = 1.473) | 440 | − (2) | 520 | 80 (7) |
| ScaleView A2 (n = 1.380) | 444 | 34 (5) | 424 | 0 (1) |
Spheroids were seeded at 1000 cells per well and grown for 4 or 5 days. The depth varied with dyes: Propidium Iodide and Hoechst 33342. Microscopy was performed on the Olympus FV1000 confocal microscope.