Fig. 2. aaD in primary adult rat hepatocytes induces a quiescence state, which can be reversed by aa addition.

Cell counting of primary adult rat hepatocytes at t0, 24 h and 48 h after isolation. For the last time point, cells were kept in presence or absence of aa (DMEM; w/o aa) for 48 h, or in medium w/o aa for 24 h with the restoration of total aa a or essential aa b for the following 24 h. Values are means ± SEM (n = 3). *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001