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. 2019 Jul 22;10(8):566. doi: 10.1038/s41419-019-1779-7

Fig. 4. Essential aaD significantly increases Drosha protein in primary adult rat hepatocytes.

Fig. 4

a, b Western blot quantification a and analysis b of Drosha protein level after 24 h in a full-aa medium (DMEM) or in medium w/o aa. Values are means ± SEM (n = 5). ***p < 0.0005. c, d Western blot quantification c and analysis d of Drosha protein level after 48 h in DMEM or medium w/o aa. Values are means ± SEM (n = 16). ****p < 0.0001. e, f Drosha mRNA measurement in primary adult rat hepatocytes: after 24 h e or 48 h f aaD, RNA was extracted, reverse transcribed and analyzed by quantitative PCR. Gene expression was normalized to the cyclophilin A (CyA) mRNA level. Values are means ± SEM (n = 3). g, h Western blot quantification g and analysis h of Drosha protein level after 48 h in DMEM, w/o aa and in medium w/o aa supplemented with essential aa (w/o aa + e.aa). Values are means ± SEM (n = 7). ****p < 0.0001