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. 2019 Jul 22;10(8):566. doi: 10.1038/s41419-019-1779-7

Fig. 7. Drosha knockdown restores cell proliferation in aa-deprived primary adult hepatocytes without affecting oxygen consumption and autophagy.

Fig. 7

a, b Cell counting of primary rat hepatocytes transfected with 50 nmol/L negative control-siRNA (Neg. Ctr) or Drosha-siRNA (siDrosha) for 24 h a and 48 h b, in full-aa medium (DMEM) or in absence of aa (w/o aa). Values are means ± SEM (n = 3). *p < 0.05, ***p < 0.0005. c, d Mitochondrial oxygen consumption rate (OCR) c and basal extracellular acidification rate (ECAR) d from primary rat hepatocytes cultured in DMEM or w/o aa and transfected with 50 nmol/L negative control-siRNA (Neg. Ctr) or Drosha-siRNA (siDrosha) for 48 h. Maximal OCR was determined using FCCP (2 µm). OCR is reported in the unit of picomoles per minute and ECAR is reported in milli-pH units (mpH) per minute. Values are means ± SEM (n = 11 replicates from two independent rats). **p < 0.005. e–h Western blot quantification and analysis of LC3-I/II in primary adult rat hepatocytes transfected with 50 nmol/L negative control-siRNA (Neg. Ctr) or Drosha-siRNA (siDrosha) and kept in culture for 24 h e, f or 48 h g, h either in DMEM or in medium w/o aa; cells were treated with 25 µm chloroquine for 1 h or 4 h before protein extraction. Values are means ± SEM (n = 3). *p < 0.05