Skip to main content
. 2019 Jul 16;10:587. doi: 10.3389/fgene.2019.00587

Figure 5.

Figure 5

Schematic representation of the different expression vectors used in this study and confirmation of genomic integration and protein expression. (A) Structure of the expression vectors used to establish the K4D and K4DT + T cell lines. LTR, long terminal repeat; CMV, cytomegalovirus promoter; Hygro, Hygromycin-resistant gene. (B) Confirmation of the genomic integration of the TERT expression cassette by PCR: (1) primary cells; (2) K4D cells; (3) K4DT + T cells. The upper panel shows the specific 500-bp PCR product confirming the presence of the TERT expression cassette, whereas the lower panel shows the 400-bp PCR product derived from the human Tuberous Sclerosis Type II gene.