Table 1.
Primers used for detection of genes for virulence factors in E. faecium strains isolated from rhizosphere.
| Virulence gene | Primer sequence | Amplicon size | References |
|---|---|---|---|
| agg | F:AAGAAAAAGAAGTAGACCAAC R:AAACGGCAAGACAAGTAAATA |
153 | Eaton and Gasson, 2001 |
| ccf | F:GGGAATTGAGTAGTGAAGAAG R: AGCCGCTAAAATCGGTAAA |
543 | Eaton and Gasson, 2001 |
| cob | F: AACATTCAGCAAACAAAGC R:TTGTCATAAAGAGTGGTCAT |
1405 | Eaton and Gasson, 2001 |
| cpd | F:TGGTGGGTTATTTTTCAATTC R: TACGGCTCTGGCTTACTA |
782 | Eaton and Gasson, 2001 |
| cyl | F: ACTCGGGGATTGATAGGC R: GCTGCTAAAGCTGCGCTT |
688 | Vankerckhoven et al., 2004 |
| efaAfm | F:AACAGATCCGCATGAATA R: CATTTCATCATCTGATAGTA |
735 | Eaton and Gasson, 2001 |
| esp | F:AGATTTCATCTTTGATTCTTGG R:AATTGATTCTTTAGCATCTGG |
510 | Vankerckhoven et al., 2004 |
| gelE | F:ACCCCGTATCATTGGTTT R: ACGCATTGCTTTTCCATC |
419 | Sabia et al., 2008 |