Table 2.

List of Aspergillus proteins detected by mAb AP3.

Spot no.	Short name	Protein	NCBI reference sequence	MW (kDa)	pIa	Scoreb	Peptidesc	Sequence coverage (%)	Signal peptided	N-glycan acceptor sitese	O-glycan acceptor sitesf	Cellular localization	Functiong
3	Catalase	Mycelial catalase cat1	XP_002380889.1	79.8	5.34	398	18 (13)	21	Yes	4	24	Extracellular (cell wall)	Cell protection (Paris et al., 2003)
4						373	14 (9)	17
6	Hsp70	Hsp70 chaperone Hsp88	XP_002381416.1	79.8	5.02	680	32 (23)	34	No	3	3	Intracellular	Protein folding (Teutschbein et al., 2010)
8						331	16 (10)	19
7	Hsp90	Molecular chaperone and allergen Mod-E/Hsp90/Hsp1	XP_002382894.1	79.6	4.97	405	13 (10)	20	No	4	8	Intracellular (cytosolic, cell wall)	Protein folding (Lamoth et al., 2012, 2014, 2016)
9	Amidase	Amidase family protein	XP_002377652.1	60.7	5.22	276	11 (7)	22	Yes	7	9	Extracellular	Unknown
13	Glucanase	Cell wall glucanase (Scw11), putative	XP_002372749.1	61.8	4.63	155	6 (4)	10	Yes	0	122	Extracellular (cell wall)	Cell wall remodeling (Mouyna et al., 2013)
14						262	10 (6)	15
15						187	8 (5)	11

Protein spots were identified by Tandem mass spectrometry and protein sequences were analyzed in silico using the A. flavus NRRL3357 reference database.

^aPredicted Mw and pI by ExPASy Compute pI/Mw tool.

^bProtein score in Mascot Search.

^cNumber of total identified peptides/unique peptides.

^dPredicted signal peptide (ProP 1.0).

^ePredicted N-glycosylation sites (NetNGlyc1.0).

^fPredicted O-glycosylation sites (NetOGlyc4.0).

^gReference.