Figure 2.

One-dimensional excitation (blue) and emission (red) spectrum of ANNINE-6 measured in a neuronal membrane (Kuhn and Fromherz, 2003). The intensity of the excitation spectrum was measured at the peak of the emission spectrum (565 nm). The excitation spectrum acts here as a proxy for the absorption spectrum mentioned in the text. To measure the emission spectrum ANNINE-6 was excited at the peak of the excitation spectrum (420 nm). Both spectra are normalized.