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. 2019 Jul 22;9:10568. doi: 10.1038/s41598-019-47122-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Real-time analysis of micro-irradiated AB nucleus revealed the subcellular relocalization of HUS-1::GFP (foci). First cell divisions of 2-cell stage embryos are observed using a strain expressing HUS-1::GFP (opls34). Before irradiation HUS-1::GFP is homogenously distributed in nuclei (t = 0 min). The AB cell nucleus is targeted with protons at t = 0 min. In micro-irradiated AB nucleus of HUS-1::GFP embryo, a focus, indicated with white arrow (→), appears just before the first cell division of AB (t = 2 min.) and reappears in the daughter cells ABa and ABp indicated with stars (*, right). We never observed foci in both non-irradiated embryo (control) and neighbouring non-irradiated nuclei (P1, EMS, and P2). Irradiated embryos (opls34 = 10⁴ protons) were observed in real time following irradiation. Scale bar: 10 µm.