Figure 5.

GFAP immunoreactivity in the midbrain of sham and BLG-sensitized mice. GFAP-positive astrocytes were identified by immunohistochemically staining brain sections (40 μm). Representative midbrain sections from sham (A,a',a”) and BLG-sensitized (B,b',b”) male mice are shown. The white rectangles in (A,B) indicate where the respective higher magnification images a'–b” were taken. The open arrowheads indicate dense GFAP-immunoreactive astrocyte end-feet along the blood vessel. The low-magnification (A,B) and high-magnification (a',a”, b',b”) images taken with a 10X and 40X objectives, respectively. Scale bars: 100 μm for (A) and (B); 25 μm for a'–b”.