Figure 7.

Western blot analysis of COX-2 in the isolated five brain regions. Soluble proteins isolated from the five regions were resolved on discontinuous 15% SDS-polyacrylamide gels for western blot detection of COX-2 (upper panels). (A) Region 1, (B) Region 2, (C) Region 3, (D) Region 4, and (E) Region 5 as described in Figure 1B. Chemiluminescence signals for COX-2 were digitally captured and shown in the upper panels. GAPDH was also detected from the same blots and used as a reference for loading variability (lower panels). The captured COX-2 signals were quantified using LI-COR Image Studio Lite software and normalized to GAPDH signals. Values shown in the bar graphs indicate the group average ± SEM. **p < 0.001 (unpaired t-test), n = 8.