Figure 1.

Measurement of oxidative burst and phagocitosis in granulocytes with PHAGOBURST^TM test (Glycotope Biotechnology GmbH, Heidelberg, Germany) by flow cytometer. A representative example of functional granulocyte activity which produced different amount of reactive oxidants in patient with mutation in ARPC1B (A) and healthy control (B). Red – unstimulated granulocytes; blue – fMLP stimulated granulocytes; orange – PMA stimulated granulocytes. Different phagocytic activity of granulocytes from patients with mutation in ARPC1B (C) and healty control (D) after phagocytosis of E. coli (blue histogram) and negative control (red histogram).