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. 2019 Jul 16;10:894. doi: 10.3389/fpls.2019.00894

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Copyright © 2019 Liu, Atanasov, Tiburcio and Alcázar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Polyamine levels in response to flg22 treatment. Levels of free putrescine (Put), spermidine (Spd), and spermine (Spm) in 10-day-old wild-type, adc1-2, adc1-3, adc2-3, adc2-4 (Cuevas et al., 2008), and fls2 (Zipfel et al., 2004) seedlings treated with 1 μM flg22. Seedlings were grown in vitro on a nylon mesh in ½ Murashige and Skoog media and transferred to the same media supplemented with 1 μM flg22 or mock for 24 h. Samples were harvested after 0, 3, and 24 h of treatment for polyamine analyses. Results are mean of three biological replicates ± SD (standard deviation). Letters indicate values that are significantly different according to Student–Newman–Keuls test at P value <0.05.