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. 2019 Jul 16;10:894. doi: 10.3389/fpls.2019.00894

FIGURE 6.

FIGURE 6

Expression analyses of PTI marker genes in response to 100 μM Put in rbohD, rbohF, and double rbohD/F loss-of-function mutants. Ten-day-old wild-type, atrbohD (SALK_109396 and SALK_005253), atrbohF (SALK_044584 and SALK_077748), and double atrbohD/F seedlings were treated with 100 μM Put or mock as described in Figure 4 and samples were collected at 0 and 1 h post-treatment to analyze the expression of WRKY22 and CYP81F2 by qRT-PCR. Expression values are relative to the wild type at 0 h. Results are means of three biological replicates ± SD (standard deviation). Letters indicate values that are significantly different according to Student–Newman–Keuls test at P value <0.05.