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. 2019 Apr 10;8(8):858–869. doi: 10.1002/sctm.18-0206

Figure 1.

Figure 1

Characterization of stored and not stored (fresh) mouse decellularized diaphragms. (A): Histological analyses of diaphragmatic acellular scaffolds immediately after decellularization (fresh) or after different types and timings of storing: 2 weeks at 4°C (2w4C), 2 months at 4°C (2m4C), 2 weeks in liquid nitrogen (2wLN), and 2 months in liquid nitrogen (2mLN). HE stain for general appearance of the muscle, MT for collagen, AB for GAG. Scale bar: 100 μm. (B–D): Elastin, collagen, and sGAG quantifications of decellularized diaphragms. Fresh decellularized samples were used as control. ***, p < .001. Scale bar: 100 μm. Abbreviations: HE, hematoxylin and eosin; MT, Masson's trichrome; AB, Alcian blue; GAG, glycosaminoglycans; sGAG, sulfated GAG; n.s., not significant by Student's t test.