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Localization of transplanted cells following immunostaining or retinal sections with antibodies to both human nestin plus CD29 and Alexa flour 488 used as a single secondary antibody. Images show eyes transplanted with (A) 1 × 105 cells and (B) 4 × 104 cells. White arrows indicate the location of the cells. Scale = 50 μm. Abbreviations: INL, inner nuclear layer; ONL, outer nuclear layer; RGC, retinal ganglion cell layer.
