Table 1.
Cultured and cryopreserved adipose‐derived mesenchymal stem cells reduce lung cytokine levels during pneumosepsis
| 16 hours | 48 hours | |||
|---|---|---|---|---|
| Control | ASCs | Control | ASCs | |
| Cultured ASCs (pg/ml) | ||||
| TNF‐α | 2,154 (1,718–3,411) | 1,384 (1,061–1,655)**** | 2,173 (1,184–3,312) | 894 (642–1,792)*** |
| IL‐1β | 1,066 (480–1,944) | 570 (440–789)* | 767 (460–1,598) | 310 (157–534)*** |
| IL‐6 | 3,003 (1,598–3,858) | 1,405 (1,136–2,658)* | 874 (366–1,646) | 361 (276–675)** |
| MIP‐2 | 15,684 (13,972–17,587) | 12,865 (9,323–15,261)* | 17,086 (12,279–26,432) | 12,233 (9,458–14,011)*** |
| Cryopreserved ASCs (pg/ml) | ||||
| TNF‐α | 2,512 (2,381–2,967) | 1,540 (1,156–1,982)* | 1,674 (1,242–2,644) | 910 (738–2,180)* |
| IL‐1β | 1,267 (1,193–1,454) | 690 (373–936)** | 665 (363–1,207) | 341 (208–696)* |
| IL‐6 | 4,084 (3,812–5,423) | 1,951 (1,410–3,174)** | 2,322 (777–5,531) | 1,733 (912–2,534) |
| MIP‐2 | 20,874 (19,257–22,821) | 17,383 (16,809–18,654)** | 18,331 (13,574–32,766) | 12,928 (9,946–19,983)* |
Mice were treated with 1 × 106 ASCs intravenously either 1 or 6 hours after infection with Klebsiella pneumoniae via the airways and lungs were harvested 16 or 48 hours after infection, respectively. Data are median (interquartile range) of 8–24 mice per group.
*
p < .05.
**
p < .01.
***
p < .001.
****
p < .0001 versus control.
Abbreviations: ASCs, adipose‐derived mesenchymal stem cells; TNF, tumor necrosis factor; IL, interleukin; MIP, macrophage inflammatory protein.