Table 2.
Cryopreserved adipose‐derived mesenchymal stem cells reduce plasma cytokine levels during pneumosepsis
| 16 hours | 48 hours | |||
|---|---|---|---|---|
| Control | ASCs | Control | ASCs | |
| TNF‐α (pg/ml) | 11 (6–92) | 5 (5–5)* | 25 (15–44) | 22 (9–28) |
| IL‐6 (pg/ml) | 80 (53–175) | 33 (17–41)** | 155 (75–358) | 62 (23–137)** |
| MCP‐1 (pg/ml) | 204 (65–1,797) | 34 (31–58)* | 375 (159–537) | 189 (64–15–254)* |
| E‐selectin (ng/ml) | 605 (401–965) | 645 (525–864) | 370 (306–404) | 396 (278–434) |
| VCAM‐1 (pg/ml) | 1,201 (1,075–1,517) | 1,202 (1,032–1,403) | 1,290 (1,034–1,925) | 1,575 (1,315–1,862) |
| ALT (pg/ml) | n.d. | n.d. | 62 (13–198) | 34 (11–72) |
Mice were treated with 1 × 106 cryopreserved ASCs intravenously either 1 or 6 hours after infection with Klebsiella pneumoniae via the airways and lungs were harvested 16 or 48 hours after infection, respectively. Data are medians (interquartile range) of 8–24 mice per group.
*
p < .05.
**
p < .01 versus control.
Abbreviation: ASCs, adipose‐derived mesenchymal stem cells; TNF, tumor necrosis factor; IL, interleukin; MCP, monocyte chemotactic protein; VCAM, vascular cell adhesion molecule; ALT, alanine aminotransferase; n.d., not determined.