Figure 1.

Methods for identifying CWGTs for in vitro activity verification. The activities of plant cell wall glycosyltransferases have been identified using three main strategies as outlined in this review: activity enrichment, mutational genetics, and omics-directed GT selection. Heterologous expression and in vitro assays are critical steps in the process of verifying the synthesis of specific cell wall linkages associated with different families of CWGTs. Purification of enzymes following heterologous expressional enables advanced studies of GT mechanism, including enzyme kinetic, and polysaccharide chain length analyses.