Table 1.
The primer sequences used for the PCR-based amplification of the VAGs in Escherichia coli isolated from UTI patients
| Gene | Amplicon size (base pairs) | Primer sequence | Reference |
|---|---|---|---|
| cvaC | 1181 | F TGGTAGAATGTGCCAGAGCAAG R GAGCTGTTTGTAGCGAAGCC |
16 |
| fimH | 419 | F TTTTGCGACAGACCAACAACTAT R TTGCACATTCCCTGCAGTCAC |
4 |
| papC | 347 | F GGCGTGATAACGATTCTGCTTACCT R GGACATTCCCCCTGCATGTAAC |
13 |
| papG allele I | 461 | F TCGTGCTCAGGTCCGGAATTT R TGGCATCCCCCAACATTATCG |
14,16 |
| papG allele II | 190 | F GGGATGAGCGGGCCTTTGAT R CGGGCCCCCAAGTAACTCG |
14,16 |
| papG allele III | 258 | F GGCCTGCAATGGATTTACCTGG R CCACCAAATGACCATGCCAGAC |
14,16 |
| sfa | 410 | F CTCCGGAGAACTGGGTGCATCTTAC R CGGAGGAGTAATTACAAACCTGGCA |
14 |
| cnf-1 | 498 | F AAGATGGAGTTTCCTATGCAGGAG R TGGAGTTTCCTATGCAGGAG |
14 |
| hlyA | 1177 | F AACAAGGATAAGCACTGTTCTGGCT R ACCATATAAGCGGTCATTCCCGTCA |
4,14,15 |
| draD | 407 | F ATGAACGGGAGTATAAGGAAGA R AACCGGTATTCACCAGGAGCAA |
4 |
| kpsM | 454 | F GCGCATTTGCTGATACTGTTGGG R GAGGGAACATGATGCAGGAGATG |
4 |
| neuA | 474 | F CTTCACCCTTTAGAGATTCGACCC R CCCAATAATCAAACAGCGAGTGTCC |
4 |
| sat | 465 | F ACAGCCTGAAACTGAAACACCGAC R ACATCACTGAAGCCAATACTCC |
4,12 |
| mcmA | 278 | F TAACTTCCACTCCCCGCA R ATGAGAAAACTATCTGAAAATGAAAT |
13 |
| mchB | 228 | F ATGCGAGAAATAACAGAATCACAG R TTAGCTACCGCCACCAGCAGAAG |
13 |
| mciA | 234 | ATGAGAGAAATATCAGATAACATGCTTG TTAACTGCCGCTGTTTGCACTG |
13 |
| mceA | 300 | ATGAGAGAAATTAGTCAAAAGGAC TTAACTACCACTACCGGAAC |
13 |
Abbreviations: F, forward; R, reverse; UTI, urinary tract infection; VAG, virulence-associated gene.