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. 2019 Jul 18;12:5835–5848. doi: 10.2147/OTT.S207211

Figure 5.

Figure 5

GLUT1 knockdown restrains PKC-iota-mediated growth of NSCLC cells. (A) After co-transfection with Flag-PKC-iota (or empty vector) and Si GLUT1 (or Si NC), the numbers of H1650 cells were counted at 24, 48, and 72 h. (B, C) H1650 cells were co-transfected with Flag-PKC-iota (or empty vector) and Si GLUT1 (or Si NC) for 36 h, after which time the cells were harvested to determine the migration and invasion capabilities by the wound-healing assay (left of (B) images of the indicated cells, scale bar: 25 μm; right of (B) statistical analysis of the migratory ability of the indicated cells) and transwell migration assay (left of (C) images of the indicated cells, scale bar: 25 μm; right of (C) statistical analysis of the invasive ability of the indicated cells), respectively.