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. 2019 Jul 17;9:43–57. doi: 10.2147/PTT.S200366

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© 2019 Agarwal et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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Non-linear microscopy of dermal region of skin tissue for healthy and treated biopsies: display of forward CARS, epi SHG, and epi TPEF, overlay of CARS, SHG, and TPEF, and bright-field images in the dermis region of the skin for each sample condition. Row 1: lesional psoriasis tissue before treatment; row 2: psoriasis tissue treated with NBUVB; row 3: healthy tissue; and row 4: healthy tissue treated with NBUVB. The bright-field images indicate the dermis (green line). Scale bar: 20 µm.

Abbreviations: CARS, coherent anti-Stokes Raman spectroscopy; NBUVB, narrowband ultraviolet B; SHG, second-harmonic generation microscopy; TPEF, two-photon excitation fluorescence microscopy.