Figure 5.

SPRY4-IT1 Serves as a Sponge for miR-101-3p in GC

(A) StarBase version (v.)2.0 results showing the sequence of SPRY4-IT1 with highly conserved putative miR-101-3p-binding sites. (B) miR-101-3p mimic considerably reduced the luciferase activity of the WT-SPRY4-IT1 luciferase reporter vector compared with negative control, while SPRY4-IT1 mimic did not have any impact on the luciferase activity of MUT-SPRY4-IT1-transfected cells. (C) SPRY4-IT1 and miR-101-3p simultaneously existed in the production precipitated by anti-AGO2. (D) The level of miR-101-3p was significantly decreased in GC tissues as compared to that in matched non-tumor tissues. (E) Silencing of SPRY4-IT1 increased the expression level of miR-101-3p in BGC823 cells. All tests were performed at least three times. Data were expressed as mean ± SD. **p < 0.01.