Figure 2.

Y‐27632 induced significant dose‐dependent inhibition of human Tenon fibroblast cell‐mediated collagen gel contraction. The dose dependence of collagen gel contraction was tested with various concentrations of antiglaucoma drugs (latanoprost and timolol maleate), Y‐27632, and TGF‐β, from 0μM through 100μM, for 24 h. A, Images show representative pictures of the collagen gel. B, Contraction was significantly reduced by a minimum treatment of 5μM Y‐27632; it reached a maximum at 100μM Y‐27632. Data represent the mean ± SD (n = 4) and were analysed by one‐way ANOVA, followed by the Dunnett post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001. ANOVA, analysis of variance; TGF‐β, transforming growth factor‐β