Figure 4.

Effect of Y‐27632 on TGF‐β–induced fibrosis. A, HTFs were cultured on collagen gels before treatment with Y‐27632, timolol, and latanoprost for 24 h; HTFs were then incubated with TGF‐β (top) and without TGF‐β (bottom) for 1 day to mimic postoperative excretion of TGF‐β and to assess the ability of Y‐27632 to suppress the TGF‐β effect. The extent of contraction was then measured. Data shown are the mean ± SD; *P < 0.001 by one‐way ANOVA, followed by the Dunnett post hoc test. B, Lysates of collagen gels were prepared for western blotting with antibodies to α‐SMA, vimentin, and tubulin (loading control). Data are representative of three independent experiments performed in triplicate (NS: nonsignificant). ANOVA, analysis of variance; HTF, human Tenon fibroblast; TGF‐β, transforming growth factor‐β; α‐SMA, α‐smooth muscle actin