Figure 5.

ROCK inhibitor decreased latanoprost‐, timolol‐, and combination latanoprost/timolol–induced collagen gel contraction and expression of α‐SMA and vimentin. A, HTFs were serum‐starved overnight, stimulated with antiglaucoma drugs for 24 h, and then treated with and without Y‐27632 afterwards. Diameter changes of gel contraction were observed 24 h after Y‐27632 stimulation. B, Gels were then lysed and analysed by western blotting. Blots were reprobed for tubulin as a loading control. The statistical significance of differences between groups treated and untreated with Y‐27632 was determined by the Student unpaired t test (n = 6). Differences were considered statistically significant when *P < 0.05 (NS: nonsignificant). HTF, human Tenon fibroblast; ROCK, Rho‐associated protein kinase; α‐SMA, α‐smooth muscle actin