Table 1.
Interassay imprecision forserum QCs.
| Validation (n = 120) |
Multiinstrument validationa (n = 75) |
||||
|---|---|---|---|---|---|
| Analyte | Level | Mean concentration, μg/mL | CV, % | Mean concentration, μg/mL | CV, % |
| Acetylthreonine | Low | 0.0774 | 5.2 | 0.0775 | 5.8 |
| Mid | 0.892 | 5.1 | 0.867 | 5.5 | |
| High | 1.78 | 4.8 | 1.69 | 4.8 | |
| Phenylacetylglutamine | Low | 0.329 | 5.2 | 0.337 | 4.2 |
| Mid | 8.17 | 4.0 | 8.30 | 4.5 | |
| High | 16.0 | 3.8 | 16.1 | 4.8 | |
| Pseudouridine | Low | 1.27 | 5.2 | 1.30 | 6.9 |
| Mid | 16.4 | 4.2 | 16.5 | 5.7 | |
| High | 32.1 | 3.7 | 31.7 | 5.6 | |
| Tryptophan | Low | 4.08 | 6.4 | 4.13 | 4.6 |
| Mid | 44.7 | 4.3 | 44.6 | 4.0 | |
| High | 88.0 | 4.3 | 87.3 | 4.7 | |
a
Data reported are from all 3 LC-MS/MSsystemstested.