Fig. 7.

AC prevents HFD-induced alterations in mouse cecal microbiota. Mice were fed a control diet (red dots, empty bars), the control diet supplemented with 40 mg AC/kg body weight (green dots, dashed bars), a HFD (blue dots, black bars), or the HFD supplemented with 40 mg AC /kg body weight (violet dots, blue bars) for 8 weeks. A- Changes in cecum microbiota were assessed by clustering of samples based on diet. NMDS was performed based on the weighted UniFrac distance matrix generated from sequencing fecal 16S rRNA gene The X-axis represents the tertiary coordinate, the Y-axis represents the secondary coordinate. Axis numbering represents the relative distance between samples based on the weighted UniFrac distance matrix. B- Ratio of fecal Firmicutes/Bacteroidetes abundance, and C- fecal Akkermansia relative abundance. B,C- Results are shown as mean ± SE of 6–10 animals/treatment. Values having different superscripts are significantly different (p < 0.05, One-way ANOVA test). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)