Table 2. Experimental design matrix using RSM with CCD and responses for the mycelial biomass (DCW), EPS and IPS production from the mycelium of G. lucidum strain QRS 5120.

Run No.	Variables				Responses
	Initial pH	Glucose (g/L)	Agitation (rpm)		Biomass (DCW g/L)		EPS (g/L)		IPS (g/L)
					Actual	Predicted	Actual	Predicted	Actual	Predicted
1	4	10	90		4.6	4.52	2.2	2.31	1.2	1.28
2	6	50	110		3.8	3.92	1.2	1.13	0.2	0.14
3	6	50	90		3.1	2.97	0.9	0.91	0.2	0.25
4	5	30	100		4.1	4.12	1.1	1.31	0.8	0.85
5	5	30	90		3.9	3.6	1.3	1.15	0.7	0.73
6	5	50	100		3.8	3.96	1	0.91	0.9	0.83
7	5	30	100		4.2	4.12	1.4	1.31	0.9	0.85
8	4	50	110		5.1	4.79	2.1	2.18	1.4	1.53
9	5	30	100		3.8	4.12	1	1.31	0.9	0.85
10	4	50	90		4.9	5.04	1.9	1.96	1.5	1.44
11	5	30	100		3.9	4.12	1.3	1.31	0.7	0.85
12	5	30	110		4.2	4.3	1.4	1.37	0.9	0.77
13	5	30	100		4.2	4.12	1.5	1.31	0.8	0.85
14	5	30	100		4.1	4.12	1.2	1.31	0.8	0.85
15	4	30	100		5.2	5.26	2.9	2.61	1.7	1.57
16	6	10	110		4.1	4	1.2	1.18	0.1	0.18
17	6	30	100		4	3.74	1.3	1.41	0.3	0.33
18	4	10	110		4.8	4.97	2.5	2.53	1.5	1.47
19	5	10	100		4.1	3.74	1.2	1.11	0.8	0.77
20	6	10	90		2	2.35	1	0.96	0.3	0.19