Figure 1.

miR-34c expression was significantly decreased, while HMGB1 expression was significantly increased in NSCLC. (A) Examples of NSCLC tissues stained with H&E. Magnification, ×200, bar =50 μm. (B) HMGB1 expression was assessed by IHC assays performed with a specific HMGB1 antibody. Magnification, ×200, bar =50 μm. (C) The relative levels of miR-34c expression in NSCLC and corresponding normal tissues were examined by the qRT-PCR assay. (D and E) The relative levels of HMGB1 protein and mRNA expression in NSCLC and corresponding normal tissues were examined by Western blot and qRT-PCR assays, respectively. (F) The levels of miR-34c in one normal human lung cell line (MRC-5) and five human NSCLC cell lines (A549, H460, H157, H1299, and H23), *P<0.05, **P<0.01 vs MRC5 cells.

Abbreviation: NSCLC, non-small cell lung cancer.