Table 4.
mRNA expression in perigonadal adipose tissue of mice offspring at 19 weeks of agea.
|
In utero environment and obesogenic group | ||||||
|---|---|---|---|---|---|---|
| VDS |
VDD |
VDS |
VDD |
VDS |
VDD |
|
| a/a-LFD | a/a-LFD | Avy/a LFD | Avy/a LFD | a/a-HFD | a/a-HFD | |
| Pparg | 9.0 ± 0.5 | 6.0 ± 0.6b | 7.9 ± 0.6 | 5.7 ± 0.7b | 6.3 ± 0.5 | 6.6 ± 0.4 |
| Cebpa | 5.7 ± 1.0 | 4.0 ± 0.9 | 4.2 ± 1.0 | 3.0 ± 0.9 | 3.5 ± 0.9 | 3.4 ± 0.8 |
| Vdr | 12.2 ± 0.6 | 10.8 ± 0.5 | 12.3 ± 0.6 | 11.3 ± 0.6 | 11.4 ± 0.8 | 11.7 ± 0.6 |
| Lep | 6.3 ± 0.8 | 4.8 ± 0.8 | 4.8 ± 0.9 | 3.0 ± 0.6 | 3.9 ± 0.8 | 3.4 ± 0.4 |
| Adip | 0.77 ± 0.4 | 0.84 ± 0.4 | 0.18 ± 0.3 | 0.67 ± 0.4 | 0.61 ± 0.3 | 0.39 ± 0.5 |
a
Data are presented as means ± s.e.m.; value shown is ΔCt (gene of interest − housekeeping gene), therefore lower values indicate higher expression
b
significantly different than value to the left (between in utero environment; P < 0.05).
a/a, non-agouti (c57BL/6); Adip, adiponectin; Avy/a, viable yellow agouti; Cebpa, CCAAT/enhancer-binding protein-alpha; HFD, high-fat diet; IU, in utero; Lep, leptin; LFD, low-fat diet; Pparg, peroxisome proliferator-activated receptor gamma; OG, offspring group (genetic background × postnatal environment); VDD, vitamin D deficient; Vdr, vitamin D receptor; VDS, vitamin D sufficient.