Fig. 3.

All panels illustrate the fabricated bacterial model membrane GUVs possessing phospholipids and LPS in the inner and outer leaflets, respectively. (a) The longevity and size distribution of our three model membrane designs for the oil extraction process. (b) Uniform vesicles containing 40 mol% cholesterol embedded in the lipid bilayer. (c) and (d) are fluorescent images of NBD-labeled DOPG/DPPE (1:1 mol%) and Alexa Flour™ 568-labeled LPS molecules assembly on the inner and outer leaflets, respectively. (e) The ultrathin double emulsion templates (ii) undergoing the partial dewetting transition after adding the ethanol solution to the extracellular domain. Red arrows point at the fully packed lipid bilayer membrane side of the double emulsions. The other droplets were excess oil drops present in the domain. (f) After introducing ethanol to the OA solution, the oil shell in model membrane (iii) ruptured into micro domains protruding outward from the surface. All scale bars denote 200 μm.